2011年4月，我室纳米材料与化学研究部俞书宏教授研究组在ACS NANO上发表题为Selective Chromogenic Detection of Thiol-Containing Biomolecules Using Carbonaceous Nanospheres Loaded with Silver Nanoparticles as Carrier的论文。

Source: ACS NANO Volume: 5 Issue: 4 Pages: 3166-3171 Published: APR 2011
Hu B (Hu, Bo)1, Zhao Y (Zhao, Yang)1, Zhu HZ (Zhu, Hai-Zhou)1, Yu SH (Yu, Shu-Hong)1
Addresses:
1. Univ Sci & Technol China, Sch Chem & Mat, Hefei Natl Lab Phys Sci Microscale, Div Nanomat & Chem, Hefei 230026, Peoples R China
E-mail Addresses: shyu@ustc.edu.cn

Reprint Address: Yu, SH (reprint author), Univ Sci & Technol China, Sch Chem & Mat, Hefei Natl Lab Phys Sci Microscale, Div Nanomat & Chem, Hefei 230026, Peoples R China

Abstract: Thiol-containing biomolecules show strong affinity with noble metal nanostructures and could not only stably protect them but also control the self-assembly process of these special nanostructures. A highly selective and sensitive chromogenic detection method has been designed for the low and high molecular weight thiol-containing biomolecules, including cysteine, glutathione, dithiothreitol, and bovine. serum albumin, using a new type of carbonaceous nanospheres loaded with silver nanoparticles. (Ag NPs) as carrier. This strategy relies upon the place-exchange process between the reporter dyes on the surface of Ag NPs and the thiol groups of thiol-containing biomolecules. The concentration of biomolecules can be determined by monitoring with the fluorescence Intensity of reporter dyes dispersed In solution. This new chromogenic assay method could selectively detect these biomolecules in the presence of various other amino acids and monosaccharides and even sensitively detect the thiol-containing biomolecules with different molecular weight, even including proteins.