2011年1月，我室温龙平、姚雪彪教授研究组与人合作在NUCLEIC ACIDS RESEARCH上发表题为CPLA 1.0: an integrated database of protein lysine acetylation的论文。

Source: NUCLEIC ACIDS RESEARCH Volume: 39 Pages: D1029-D1034 Supplement: Suppl. 1 Published: JAN 2011

Liu ZX (Liu, Zexian)2,3, Cao J (Cao, Jun)2,3, Gao XJ (Gao, Xinjiao)2,3, Zhou YH (Zhou, Yanhong)1, Wen LP (Wen, Longping)2,3, Yang XJ (Yang, Xiangjiao)4,5, Yao XB (Yao, Xuebiao)2,3, Ren JA (Ren, Jian)6, Xue Y (Xue, Yu)1
Addresses:
1. Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Dept Syst Biol, Wuhan 430074, Peoples R China
2. Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China
3. Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
4. McGill Univ, Ctr Hlth, Mol Oncol Grp, Dept Med, Montreal, PQ H3A 1A1 Canada
5. McGill Univ, McGill Canc Ctr, Montreal, PQ H3A 1A1 Canada
6. Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong Peoples R China
E-mail Addresses: renjian.sysu@gmail.com, xueyu@mail.hust.edu.cn

Reprint Address: Xue, Y (reprint author), Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Hubei Bioinformat & Mol Imaging Key Lab, Dept Syst Biol, Wuhan 430074, Peoples R China

Abstract:
As a reversible post-translational modification (PTM) discovered decades ago, protein lysine acetylation was known for its regulation of transcription through the modification of histones. Recent studies discovered that lysine acetylation targets broad substrates and especially plays an essential role in cellular metabolic regulation. Although acetylation is comparable with other major PTMs such as phosphorylation, an integrated resource still remains to be developed. In this work, we presented the compendium of protein lysine acetylation (CPLA) database for lysine acetylated substrates with their sites. From the scientific literature, we manually collected 7151 experimentally identified acetylation sites in 3311 targets. We statistically studied the regulatory roles of lysine acetylation by analyzing the Gene Ontology (GO) and InterPro annotations. Combined with protein-protein interaction information, we systematically discovered a potential human lysine acetylation network (HLAN) among histone acetyltransferases (HATs), substrates and histone deacetylases (HDACs). In particular, there are 1862 triplet relationships of HAT-substrate-HDAC retrieved from the HLAN, at least 13 of which were previously experimentally verified. The online services of CPLA database was implemented in PHP + MySQL + JavaScript, while the local packages were developed in JAVA 1.5 (J2SE 5.0). The CPLA database is freely available for all users at: http://cpla.biocuckoo.org.