| 报告题目 | Fourier lifetime excitation emission matrix imaging: probing multiple cellular signaling events in whole organisms |
| 报告人 | Dr. Leilei Peng |
| 报告人单位 | Assistant Professor of Optical Sciences, University of Arizona |
| 报告时间 | 2013-05-21 |
| 报告地点 | 合肥微尺度物质科学国家实验室九楼会议室 |
| 主办单位 | 合肥微尺度物质科学国家实验室 |
| 报告介绍 | 报告摘要：
Understanding cellular and organism functions requires studying the spatially resolved dynamics of protein networks. Föster Resonant Energy Transfer (FRET) imaging has long been established as the best method for detecting protein interactions and signaling events in living systems. FRET imaging of complex protein interactions requires an imaging method that can simultaneously detect multiple interactions underlying pathway activation.
We developed a new multiplexed fluorescence lifetime imaging (FLIM) technique that utilizes the principle of Fourier multiplexing and frequency domain fluorescence lifetime analysis. The technique performs 3D FLIM imaging of up to 4 fluorophores with 4 laser lines. Moreover, the technique allows simultaneously imaging of all possible excitation-emission combination in the fluorescent sample. The complete set of lifetime and intensity excitation-emission matrix images opens the door to quantitative analysis of multi-component protein interactions.
The Fourier lifetime excitation-emission matrix (FLEEM) imaging technique forms image in two modes: the traditional confocal laser scanning mode, and a novel scanning laser tomography mode. The confocal mode is ideal for live imaging in cell cultures. However, due the complex nature of biological systems, results of in vitro cell experiments always need to be tested in in vivo animal models in order to determine relevance and discard artificial findings. To perform in vivo multiplex FRET imaging in whole organisms, we further developed a scanning laser multi-color FLIM tomography system, whose image penetration is extended to 1 mm. The tomography system is applied to live imaging of whole zebrafish embryo with up to 4 fluorescent labels. Initial results from cAMP and Ca2+ FRET senors in zebrafish embryos will be dicussed. These results demonstrated that the technique has a promising future on studying how functionality emerges from interactions between a network of basic components in cells and organisms.
报告人简介：
Dr. Leilei Peng received her B.S. and M.S. degrees in modern physics from the University of Science and Technology of China, and her Ph.D. degree in physics from Purdue University in 2005. She then went to the Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School as a research fellow. In 2008, she received a NIH Pathway to Independence Award to support her research in developing new fluorescence imaging techniques for biomedical research. She joined the faculty of the College Optical Sciences at the University of Arizona in 2009. |
