| 报告题目 | Characterization of bacterial group behavior in swimming, swarming and during biofilm formation |
| 报告人 | Dr. Rongjing Zhang |
| 报告人单位 | Rowland Institute at Harvard University |
| 报告时间 | 2013-03-13 |
| 报告地点 | 合肥微尺度物质科学国家实验室九楼会议室 |
| 主办单位 | 合肥微尺度物质科学国家实验室 |
| 报告介绍 | Abstract:
Bacteria affect hosts in groups. Depending on environment, such as nutrient and/or humidity, they swim, swarm, or form biofilms. Biofilm is responsible for over 80% of the microbial infection in human body. However, little is known about the process during the formation. Swarming is usually considered as the first stage of starting a biofilm. Swarming bacteria are grown in a rich medium on agar, have elongated cell bodies, produce more flagella, and swim in a thin film of fluid over the agar surface in swirling packs. This spreading mechanism helps cells establish infections. In this report, the thin layer of the fluid around the cells were labeled and monitored by a novel method; the properties of the fluid were measured and how this environment may help the cells to invade host was discussed. Besides starting biofilm from a surface, a new way which forms biofilm from free swimming cells in 3D space was developed. This method increases the biofilm growth rate in an order of magnitude, comparing with traditional methods. The motility change before the biofilm formation was monitored by dark field flickering microscopy (DFM), an optical method which is able to quantify up to 10^4 cells simultaneously. The significance and application of the results will be discussed as well. |
